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Data and Software Availability

Journal: Cell stem cell

Article Title: Engineered Epidermal Progenitor Cells can Correct Diet-induced Obesity and Diabetes

doi: 10.1016/j.stem.2017.06.016

Figure Lengend Snippet: Data and Software Availability

Article Snippet: A complete list of software for data analysis and processing can be found in the . table ft1 table-wrap mode="anchored" t5 KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig anti-Keratin 5 (K5) From Elaine Fuchs lab gift Guinea pig anti-Keratin 14 (K14) From Elaine Fuchs lab gift Rabbit anti-Keratin 10 (K10) From Elaine Fuchs lab gift Rabbit anti-Loricin (Lor) From Elaine Fuchs lab gift Rat monoclonal β4-integrin BD Pharmingen 553745 Ser10 pho-histone antibody EMD Millipore 06-570 Cleaved caspase-3 antibody Cell Signaling 9661 Anti-insulin Cell signaling 3014 HRP-conjugated goat anti-rabbit serum Jackson Lab 111-036-046 Chemicals, Peptides, and Recombinant Proteins Keratinocyte E base medium (DMEM:F12=3:1, without calcium) Invitrogen/Gibco Custom order FBS Omega Scientific FB-11 OCT Tissue-Tek 4583 paraformaldehyde EMS 15710 Ethanol Fischer 64-17-5 Xylene Fischer UN1307 Hematoxylin/Eosin stain Cancer Diagnostics CM3951/EM000G-P Propidium Iodide Fischer 25535-16-4 PBS Gibco 14190 SDS-polyacrylamide gel National Diagnostics EC-890 Odyssey blocking buffer Li-Cor 921-40000 doxycycline Sigma D9891 glucose Sigma D9434 insulin Sigma 91077C Ingenio electroporation solution Mirus Bio LLC MIR 50111 Mitomycin C Santa Cruz Sc-3514 Critical Commercial Assays Insulin ELISA kit EMD Millipore Corp EZRMI-13K GLP-1 ELISA kit Sigma RAB0201-1KT SignalStain DAB substrate kit Cell signaling 8059 Experimental Models: Cell Lines Mouse: primary epidermal basal cells This paper N/A Human: primary epidermal basal cells Invitroge C0015C Experimental Models: Organisms/Strains Mouse: CD-1 Transgenic Core Facility/University of Chicago N/A Mouse: Nude Jackson Laboratories Stock 007850 Software and Algorithms Pannoramic Viewer 1.15 3DHISTECH ltd. www.3dhistech.com/pannoramic_viewer ImageJ NIH https://imagej.nih.gov/ij/ FlowJo FlowJo LLC https://www.flowjo.com/ Excel Microsoft https://products.office.com/en-us/excel OriginLab OriginLab www.originlab.com Open in a separate window Data and Software Availability

Techniques: Software, Recombinant, Staining, Blocking Assay, Electroporation, Enzyme-linked Immunosorbent Assay, Transgenic Assay